ADIPOQ anticorps

N° du produit ABIN6251811

Cet anticorps Souris Monoclonal détecte spécifiquement ADIPOQ dans ELISA, IHC, FACS et IF. Il présente une réactivité envers Humain.

Aperçu rapide pour ADIPOQ anticorps (ABIN6251811)

Antigène: ADIPOQ (Adiponectin (ADIPOQ))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ADIPOQ est non-conjugé

Application: ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: ADPQ-1

Détail du produit anti-ADIPOQ anticorps

Purification: Protein G affinity chromatography

Immunogène: A human partial recombinant protein was used as the immunogen for this Adiponectin antibody.

Isotype: IgG2b

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/10^6 cells

IF: 1-2 μg/mL

ELISA: order BSA free format for coating

IHC (FFPE): 1-2 μg/mL (1)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Adiponectin antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissue requires boiling sections in 10  mM citrate buffer,  pH 6, for 10-20 min followed by cooling at RT for 20 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur ADIPOQ

Antigène: ADIPOQ (Adiponectin (ADIPOQ))

Autre désignation: Adiponectin

Sujet: Adiponectin (commonly called ADIPO, or by the gene name ADIPOQ) is an adipocytokine, a hormone produced in adipose tissue. It is abundantly present in plasma and has insulin like effect on glucose levels in the blood. Plasma ADIPO levels are low in insulin resistant patients who are obese, have diabetes mellitus type 2 or HIV-lipodystrophy. In women, levels tend to be higher than in men, which may be due to androgens suppressing ADIPO levels. Furthermore, adiponectin and leptin are both indicated in regulating body weight through direct action on the hypothalamus, influencing appetite. Obese people have low ADIPO levels while levels in anorexia patients are high. Adiponectin acts as ligand for various receptors, two of which have been identified, one probably involved in carbohydrate assimilation, the other in tuning the rate of metabolism.

ID gène: 9370

UniProt: Q15848